- Narrator as Detective Herlock Solmes
- Frederick Bronski as Mario
- Anna Bronski as Pauline
- S.S. Colonel Erhardt as Luigi
- S.S. Captain Schultz as Toad
- Lieutenant Andrei Sobinski as Oogtar
- Professor Siletski as Yoshi
- Sondheim as Wario
- Gruba as Birdo
- Dobish as Donkey Kong
- and more
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