- Tony Rome as Mario
- Ann Archer as Princess Peach
- Diana Pines as Princess Daisy
- Rita Klosterman as Birdo
- Rudy Klosterman as Toad
- Lt. Dave Santini as Oogtar
- Turpin as Luigi
- Boyd as Wario
- Rood as Waluigi
- Lorna as Toadette
- Catleg as Detective Herlock Solmes
- Packy as Yoshi
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