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- Tuffy Mouse (Tom and Jerry) as Basil
- Fievel (An American Tail) as Dawson
- Minnie Mouse as Olivia Flaversham
- Mickey Mouse as Hiram Flaversham
- Biana (The Rescuers) as Mrs. Judson
- Professor Ratigan as Himself
- Fidget as Himself
- Ratigan's Thug's as Themselves
- Olivia Flaversham (The Great Mouse Detective) as Barmaid
- Mouse Queen as Herself
- Lady (Lady and the Tramp) as Toby