Twilight Sparkle is the main antagonist of the notorious "grim-dark" fan-fiction known as The Experiments of Twilight Sparkle - which was inspired by other notable horror-stories such as Cupcakes as well as the Psycho Twilight meme. The story is extremely brutal and controversial, like most "grim-dark" fiction - however it also has elements of shock humor and black comedy: though the comedy value can be considered "pure black" as many would be distracted by the extreme levels of cruelty shown in the story and the further one reads the less comedic the scenario becomes as Twilight takes unrelenting joy in the suffering of her friends.
Part of what makes the story shocking is that Twilight Sparkle tortures and kills her five friends (the Mane Six) - in graphic and over-the-top ways, forcing them to watch individually as their friends are murdered before their eyes.
Twilight Sparkle sees the horrific torture and death as part of her science experiments but it is also clear she takes pleasure out of her actions - using "science" as an excuse for inflicting the most barbaric crimes upon her former friends, who are powerless to stop her.
For obvious reasons we will not go into detail on the methods used to kill each victim but each death is painful and prolonged - she even goes as far as breaking Rainbow Dash's mind when she gets tired of "cliched" torture and seeks to test out psychological abuse instead.. showing how sadistic she is in this story. She also had spike to see What she had done and what she was going to with him, Followed by Demonic\Satanic Laughter at the end. It is Unkown of what she did with him. Fans say it was something Demonic inside her,But twiligth has never been revealed to be a DEMON but maybe, just Maybe.
Type of villain-Mad Scientist,Torturer,Mind-Breakers,Sadists,Demon