- Captain Hook (from Jake and the Never Land Pirates) as Phineas Flynn
- Mr. Smee (from Jake and the Never Land Pirates) as Ferb Fletcher
- Daisy (from Thomas and Friends) as Candace Flynn
- Janja (from The Lion Guard) as Perry the Platypus
- SpongeBob SquarePants (from SpongeBob SquarePants) as Dr. Doofenshmirtz
- Red Jessica (from Jake and the Never Land Pirates) as Isabella Garcia-Shapiro
- Oscar the Grouch (from Sesame Street) as Baljeet
- Strong Bad (from Homestar Runner) as Buford van Stomm
- Diesel (from Thomas and Friends) as Jeremy Johnson
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