- Pamelda Bondani (from Patrol 03) as Mrs. Brisby
- Professor Mole (from Patrol 03) as Jeremy
- Jack Rabbit (from T.U.F.F. Puppy) as Nicodemus
- Verminious Snaptrap (from T.U.F.F. Puppy) as Mr. Ages
- Lighting Dust (from My Little Pony: Friendship is Magic) as Auntie Shrew
- Lord Tirek (from My Little Pony: Friendship is Magic) as The Great Owl
- Baboon (from Skunk Fu!) as Justin
- Mordecai (from Regular Show) as Jenner
- Raccoon (from The Nut Job) as Sullivan
- Were Skunk (from Regular Show) as Jonathan Brisby
- Simba (from The Lion King) as Dragon the Cat
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