- Michael "Mike" Roark as Mario
- Dr. Amy Barnes as Princess Peach
- Kelly Roark as Princess Daisy
- Emmit Resse as Luigi
- Dr. Jaye Calder as Princess Rosalina
- Police Lieutenant Edwin "Ed" Fox as Donkey Kong
- Norman Calder as Diddy Kong
- Gator Harris as Detective Herlock Solmes
- Stanford "Stan" Olber as Yoshi
- Roger Lapher as Toad
- Train Driver as Oogtar
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