• Michael "Mike" Roark as Mario
  • Dr. Amy Barnes as Princess Peach
  • Kelly Roark as Princess Daisy
  • Emmit Resse as Luigi
  • Dr. Jaye Calder as Princess Rosalina
  • Police Lieutenant Edwin "Ed" Fox as Donkey Kong
  • Norman Calder as Diddy Kong
  • Gator Harris as Detective Herlock Solmes
  • Stanford "Stan" Olber as Yoshi
  • Roger Lapher as Toad
  • Train Driver as Oogtar

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