- Mario as Rex
- Luigi as Woog
- Peach as Elsa
- Yoshi as Dweeb
- Donkey Kong as Captain Neweyes
- Diddy Kong as Vorb
- Toad as Louie
- Birdo as Cecilia
- Waluigi as Stubbs
- Bowser as Professor Screweyes
- Daisy as Dr. Bleeb
- Pikachu (from Pokemon) as Buster
- Misty (from Pokemon) as Buster's Mother
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