- David Randall as Mickey Mouse
- Dr. Cole Hendron as Donald Duck
- Joyce Hendron as Minnie Mouse
- Sydney Stanton as Horace Horsecollar
- Dr. Tony Drake as Scrooge McDuck
- Dr. George Frey as Professor Ludwig Von Drake
- Dr. Emery Bronson as Huey Duck
- Harold Ferris as Goofy
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