- Bob Wallace as Mickey Mouse
- Phil Davis as Donald Duck
- Betty Haynes as Minnie Mouse
- Judy Haynes as Daisy Duck
- Major General Tom Waverly as Proefssor Ludwig Von Drake
- Emma Allen as Clara Cluck
- Ed Harrison as Pluto
- Joe as Goofy
- Susan Waverly as Clarabelle Cow
- Train Conductor as Scrooge McDuck
- Railroad Stationmaster as Horace Horsecollar
- Doris Lenz as Ortensia
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