• Bob Wallace as Mickey Mouse
  • Phil Davis as Donald Duck
  • Betty Haynes as Minnie Mouse
  • Judy Haynes as Daisy Duck
  • Major General Tom Waverly as Proefssor Ludwig Von Drake
  • Emma Allen as Clara Cluck
  • Ed Harrison as Pluto
  • Joe as Goofy
  • Susan Waverly as Clarabelle Cow
  • Train Conductor as Scrooge McDuck
  • Railroad Stationmaster as Horace Horsecollar
  • Doris Lenz as Ortensia

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