- Big Bird as Roger Rabbit
- Miss Pig as Jessica
- Yogi Bear as Eddie V.
- Cindy Bear as Dolores
- Horton as Lt. Santino
- Mr. Men as Old Men
- Hyenas as Weasels
- Casey Jr as Benny the Cab
- Snuffy as Dumbo
- Quasimodo as Angelo
- BooBoo Bear as Baby Herman
- The Wicked Witch of the West as Judge Doom
- Penguins as Penguin Waiters
- Yakky Doodle as Donald Duck
- Daffy Duck as Himself
- Miss Honey as Betty Boop
- Hilda Hippo as Hyacinth Hippo
- Yosemite Sam as Himself
- Birds as Themself
- Ding-A-Ling Wolf as J. Thaddeus Toad
- Eeyore as Droopy
- Huckle's Mom as Lena Hyena
- Tweety Bird as Himself
- Bugs Bunny as Himself
- Huckle Cat as Mickey Mouse
- Mr. Fixit, Mr. Frumble, and Mr. Gronkle as Bullets
- Quick Draw McGraw as Singing Sword
- Sally Cat as Minnie Mouse
- Huckle's Dad as Goofy
- Lowly Worm as Pinocchio
- Hokey Wolf as Big Bad Wolf
- Baba Looey as Woody Woodpecker
- Sylvester as Himself
- The Toon Munchkins as The Toons
- Porky Pig as Himself