- Thomas as Roger Rabbit
- Samurai Jack as Daffy Duck
- Red Max as Donald Duck
- Emily as Jessica Rabbit
- Bowser as the Gorllia
- Mario as Eddie Valent
- Razorbeard as Judge Doom
- Wallace as Lutinet
- Smudger as Smart Ass
- Mr. L as Greasy
- Banzai as Wheezy
- Muttley as Psycho
- Mr. Snoops as Stupid
- Princess Peach as Doroisas
- Casey Jr. as Benny
- Island of Sodor as Toontown
- Rayman as Mickey Mouse
- Sonic as Bugs Bunny
- Snuk as Goofy
- Ivor as Woody Woodpecker
- Chris Griffin as Slyvester
- Johnny as Marvin ACME
- Globox as Baby Hurman
- Luigi as Eddie's brother
- Tigger as Big Bad Wolf
- Pooh Bear as Porkey Pig
- Robotnik as Yousitmente Sam
- Jano as Tasmanian Devil
- Globox's kids as Shoes
- Dip as Itself
- Lois Griffin as Betty Boop
- Peter Griffin as Droopy
- Stewie Griffin as Dumbo
- Meg Griffin as Tweety