Cast[]
- Dog as Thomas
- Monkey as Edward
- Ant as Henry
- Crab as Gordon
- Pig as James
- Bee as Percy
- Frog as Toby
- Duck as Duck
- Lion as Donald
- Whale as Douglas
- Bug as Oliver
- Snail as Bill
- Worm as Ben
- Big Mac (from TUGS) as BoCo
- Tiger as Daisy
- Kangaroo as Mavis
- Fly as Stepney
- Bear as Emily
- Puffa (from TUGS) as Bertie
- Homer (from The Simpsons) as Sir Handel
- OJ (from TUGS) as Rusty
- Top Hat (from TUGS) as Duncan
- Grampus (from TUGS) as Duke
- Captain Star (from TUGS) as Sir Topham Hatt
- Doc (from The Little Engine That Could) as Trevor
- Farnsworth (from The Little Engine That Could) as Hank
- Bird as Elizabeth
- Shark as Spencer
- Big Mickey (from TUGS) as Cranky
- Mr. Garrison (from South Park) as Mr. Percival
- Budgie (from Budgie the Little Helicopter) as Harold
- Sheep as Rosie
- Stacy Jones (from Shining Time Station) as Isobella
- Wario (from Mario) as Lorry 1
- Waluigi (from Mario) as Lorry 2
- Ricky Tricky (from Skipper & Skeeto) as D261
- Cat as Belle
- Muck (from Bob the Builder) as Flynn
- Frank (from TUGS) as Bash
- Eddie (from TUGS) as Dash
- The Sea Captain (from The Simpsons) as Ferdinand
- Owen Owl (from Skipper & Skeeto) as Rocky
- Lofty (from Bob the Builder) as Colin
- Ten Cents (from TUGS) as Charlie
- Cow as Flora
- Sunshine (from TUGS) as Neville
- Mario (from Mario) as Den
- Luigi (from Mario) as Dart
- Hercules (from TUGS) as Hiro
- Yoshi (from Mario) as Victor
- Chris (from Family Guy) as Kevin