- Yoshi as Mr. Conductor (Ringo Star)
- Donkey Kong as Harry Cupper
- Toad as Matt
- Luigi as Schemer
- Oogtar as Edmund
- Birdo as Stacy Jones
- Toadette as Tanya
- Peach as Gracie Jones
- Mario as Mr. Conductor (George Carlin)
- Wario as Mr. Conductor's Evil Twin
- and more
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