- Yoshi as Homer Simpson
- Donkey Kong as Barney Gumble
- Toad as Bart Simpson
- Luigi as Moe Syzlak
- Oogtar as Grandpa Abe Simpson
- Birdo as Marge Simpson
- Toadette as Lisa Simpson
- Peach as Maude Flanders
- Mario as Waylon Smithers, Jr.
- Wario as Mr. Burns
- Waluigi as Snake Jailbird
- and more
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