• Dr. Frederick Frankenstein as Mario
  • Igor as Luigi
  • The Monster as Yoshi
  • Inga as Birdo
  • Frau Blücher as Princess Peach
  • Elizabeth as Princess Daisy
  • Inspector Kemp as Toad
  • Herr Falkstein as Wario
  • Mr. Hilltop as Oogtar
  • Medical Student as Donkey Kong
  • Herr Walman as Bowser (deleted scenes)
  • Harold as Didddy Kong
  • Werewolf/ Cat Hit by Dart/ Victor Frankenstein as Waluigi (voice) (uncredited)
  • Frightened Villager as Toadsworth
  • Second Villager as Larry Koopa (uncredited)

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