• Dr. Frederick Frankenstein as Mickey Mouse
  • Igor as Goofy
  • The Monster as Scrooge McDuck
  • Inga as Daisy Duck
  • Frau Blucher as Minnie Mouse
  • Elizabeth as Clarabelle Cow
  • Inspector Kemp as Donald Duck
  • Herr Falkstein as Huey Duck
  • Mr. Hilltop as Dewey Duck
  • Medical Student as Louie Duck
  • Harold as Professor Ludwig Von Drake

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