- Dr. Frederick Frankenstein as Kermit the Frog
- Igor as Dr. Bunsen Honeydew
- The Monster as Gonzo the Great
- Inga as Janice
- Frau Blucher as Miss Piggy
- Elizabeth as Camilla the Chicken
- Inspector Kemp as Robin the Frog
- Herr Falkstein as Fozzie Bear
- Mr. Hilltop as Beauregard
- Medical Student as Rizzo the Rat
- Harold as Jim Henson
- Frightened Villager as Scooter
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