- Yugi Moto as Mario
- Joey Wheeler as Yoshi
- Tea Gardner as Peach
- Seto Kaiba as Luigi
- Maximillion Pegasus as Ludwig "Kooky" Von Koopa
- Alexis Rhodes as Daisy
- Rebecca Hawkins as Birdo
- Blair Flannigan as Rosalina
- Chazz Princeton as Dr. Mario
- Mai Valentine as Wendy "Kootie Pie" Koopa
- Tristan Taylor as Toadsworth
- Soloman Moto as Donkey Kong
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