- Bernie the Gorilla as Alvin
- Griffin Keyes as Simon
- Donald the Capuchin Monkey as Theodore
- Divina (from Paul Blart: Mall Cop) as Brittany
- Kate as Jeanette
- Mollie the Giraffe as Eleanor
- Joe the Lion as Dave
- Janet the Lioness as Samantha
- Sebastian the Wolf as Agent Suggs
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